WebNov 9, 2024 · Typically, phalloidin is used conjugated to a fluorescent dye, such as FITC, Rhodamine, TRITC or similar dyes, such as Alexa Fluor® 488 or iFluor 488. Phalloidin can be used with sample types such as … WebDouble stranded fluorescein labeled pGL2 plasmid (0.1 pg/ml) was co-injected with TRITC-dextran into the cytosol of HeLa cells and incubated under tissue culture conditions for the indicated time. Cells were fixed and fluorescence micrographs were taken to visualize the distribution of TRITC-dextran (right column) and fluorescein-labeled pGL2 ...

Phalloidin staining protocol Abcam

WebThe most common members of this group have been the tetramethylrhodamines—including the reactive isothiocyanate (TRITC) and carboxylic acid (TAMRA) derivatives—as well as the X-rhodamines. The X prefix of the X-rhodamines, ... fall between those of fluorescein and tetramethylrhodamine (Figure 1.6.3). With a peak absorption at ~520 nm, ... WebThe pH dependence of fluorescence of fluorescein isothiocyanate (FITC) conjugates could be described by a single proton dissociation (pK'a approximately 6.8). This allowed pH to be derived accurately from spectra using the simple Henderson-Hasslebach equation. FITC and TRITC conjugates were delivered to mouse macrophage lysosomes by pinocytosis. is back 4 blood free

Triple Band Excitation: DAPI-FITC-TRITC Nikon’s …

WebFITC-TRITC - The FITC-TRITC filter combination is designed for simultaneous detection of emission from the fluorochromes FITC and TRITC, or probe pairs that are spectrally … WebFigure 1 below illustrates the excitation and emission spectra of FITC under conditions of 488 nm excitation. FITC’s emission maximum is around 530 nm. This means that if we excite this molecule, there is a very high … WebIn fact, the fluorescein and rhodamine probes have been so widely used that most microscope and aftermarket filter manufacturers have specialized filter sets named after their reactive isothiocyanate intermediates (FITC … is back 4 blood deluxe worth it